Herpes viruses including Herpes Simplex (HSV), Cytomegalovirus (CMV), Varicella Zoster virus (VZV), Human Herpesvirus 6 (HHV6), and Epstein Barr Virus (EBV) are responsible for the common and significant viral infections associated with neonatal meningitis (HSV), adult meningitis (HSV or VZV), shingles (VZV), complications in solid organ transplant (CMV and EBV) and complications in bone marrow transplantation (CMV, EBV, HHV6). Researchers at the University Nebraska Medical Center (UNMC) have developed a multiplex PCR assay for the combined detection and diagnosis of many clinically relevant herpes viruses in patient samples. The assay is quantitative and will both benefit patients as wells as molecular labs where the number of test for detection will be decreased. Using PCR assay is less costly then other methods and has been effectively used since January 1, 2004.